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深入了解SEQIEL测序的质量值Read Quality(RQ)

Currently, the read quality in Sequel raw data ( subreads.bam) is a placeholder and not indicative of actual quality values. For example, its set to rq:f: 0.8 in Sequel generated subreads.bam. However, these rq values are populated later in the downstream analysis such as HGAP assembly, hence the filters are provided as part of our analysis applications – under the Advanced Analysis Parameters on SMRTlink GUI

To conclude, currently it is not possible to filter upstream on raw Sequel data based on read quality values as those are fixed to 0.8, however there are options for achieving filtered data during later part of analysis as Advanced Analysis Parameters

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