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Pacbio RS II和 Sequel的性能和应用领域差异

下面是PacbioRS II和pacbio Sequel的性能差异和应用领域,Pacbio 正在提升单个SMRT 的ZMW孔数和试剂版本,如下数据仅供参考。官方数据随时可能更新。

PacBio 测序仪外表

Pacbio RS II和 Sequel 三代测序仪
Pacbio RS II和 Sequel 三代测序仪
PacBio SMRT Cell 进化
PacBio SMRT Cell 进化. 左到右: SMRT Cell V2, SMRT Cell V3, SMRT Cell 1M.

PacBio 性能参数列表

RS II (P6-C4) Sequel
Run time up to 240 min up to 240 min
Total output ~500 Mb – 1 Gb 5 Gb – 10 Gb
Output/day ~2 Gb 20 Gb
Mean read length 10 -15 kb 10 -15 kb
Single pass accuracy ~86% ~86%
Consensus (30X) accuracy >99.999% >99.999%
# of reads ~50k ~500k
Instrument price ~$700k $350k
Run price ~$400 ~$850

PacBio RSII和Sequel应用领域列表

RS II (P6-C4) Sequel
Human Whole Genome Ok: While low bias and high consensus accuracy are good and long reads are advantageous for looking at structural variations, the cost per Gb is generally considered too high for large genomes Ok/Good: The low bias, high consensus accuracy, and long reads are advantageous for looking at structural variations/assembly; however, it is substantially more expensive per Gb than the leader for WGS, Illumina’s HiSeq X Ten.Ok/Good: The low bias, high consensus accuracy, and long reads are advantageous for looking at structural variations/assembly; however, it is substantially more expensive per Gb than the leader for WGS, Illumina’s HiSeq X Ten.
Exome Poor: Cost per Gb too high and output too low; long reads not useful as exons Poor: Cost per Gb too high and output too low; long reads not useful as exons
Small Genome Long reads combined with modest sequencing throughput needs make this application a good fit Long reads combined with modest sequencing throughput needs make this application a good fit
Targeted Good: Long reads combined with modest sequencing throughput needs make this application a good fit Good: Long reads combined with modest sequencing throughput needs make this application a good fit
Transcriptome Poor/Good: While the high cost per Gb and small number of reads per run make this an expensive solution, the long reads allow for full length transcript isoform sequencing which is not possible on short read platformsPoor/Good: While the high cost per Gb and small number of reads per run make this an expensive solution, the long reads allow for full length transcript isoform sequencing which is not possible on short read platforms Poor/Good: While the high cost per Gb and small number of reads per run make this an expensive solution, the long reads allow for full length transcript isoform sequencing which is not possible on short read platformsPoor/Good: While the high cost per Gb and small number of reads per run make this an expensive solution, the long reads allow for full length transcript isoform sequencing which is not possible on short read platforms
RNA Profiling Poor: The high cost per read makes this an expensive solution Poor: The high cost per read makes this an expensive solution
ChIP-Seq Poor: The high cost per read makes this an expensive solution Poor: The high cost per read makes this an expensive solution
Metagenomics Poor/Ok: While the high cost per read makes this an expensive solution, the long reads aid greatly in de novo genome assemblyPoor/Ok: While the high cost per read makes this an expensive solution, the long reads aid greatly in de novo genome assembly Poor/Ok: While the high cost per read makes this an expensive solution, the long reads aid greatly in de novo genome assemblyPoor/Ok: While the high cost per read makes this an expensive solution, the long reads aid greatly in de novo genome assembly
  1. 绿色=Good Fit, 黄色 = Ok Fit, 红色= Poor Fit。
  2. 鼠标放在3种颜色块上不动会显示对应信息。

主题测试文章,只做测试使用。发布者:测序百家,转转请注明出处:https://www.ngsgo.com/archives/569.html

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